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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in lots of tumors, which results in chromosome instability, and so might add to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical position in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two further splice variants were being noted but thorough nucleotide sequence facts just isn't but obtainable.
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Blocking peptides are peptides that bind precisely to your focus on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guide outlines further feasible optimization of cellular and immunoassay parameters to make sure the very best success are...
Antibodies sure to the blocking peptide not here bind towards the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, one can see which staining is unique.
Goal Data Cyclin E belongs towards the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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